Difference between revisions of "Part:BBa M50051"

 
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<partinfo>BBa_M50051 short</partinfo>
 
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This plasmid augments the natural expression of the LsrR repressor for the lsr operon. It is controlled through the induction of IPTG, allowing for close control of artificial expression.
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This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species.
  
 
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Revision as of 17:13, 11 December 2016


IPTG Inducible Plasmid for the Expression of LsrR Repressor

This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]