Difference between revisions of "Part:BBa M50021"

Revision as of 02:04, 11 December 2016

Heat-inducible d-Limonene synthase

Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase followed by transcriptional terminator to allow for heat-sensitive limonene catalysis. Composed (in order) of BBa_50017, BBa_50018, BBa_50019, and BBa_50049 (last=transcription terminator not added due to website error)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1895
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1600
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1597
Illegal SapI.rc site found at 319
Illegal SapI.rc site found at 1572

Revision as of 02:01, 11 December 2016 (view source) Tmerkel (Talk \| contribs) ← Older edit		Revision as of 02:04, 11 December 2016 (view source) Tmerkel (Talk \| contribs) Newer edit →
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	<partinfo>BBa_M50021 short</partinfo>		<partinfo>BBa_M50021 short</partinfo>

−	Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase to allow for heat-sensitive limonene ~~production~~	+	Combines heat-sensitive sigma32-induced promoter with strong RBS and d-limonene synthase followed by transcriptional terminator to allow for heat-sensitive limonene catalysis. Composed (in order) of BBa_50017, BBa_50018, BBa_50019, and BBa_50049 (last=transcription terminator not added due to website error)