Difference between revisions of "Part:BBa J100311"

 
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<partinfo>BBa_J100311 short</partinfo>
 
<partinfo>BBa_J100311 short</partinfo>
  
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We scrambled the TetR binding regions of the TetR repressible promoter (R0040). The scrambled promoter is not functional, whether or not aTc is present to free the binding sites. We will use it as a negative control when using repClone (J100205) to test the effects of modifications to the promoter.  
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We scrambled the TetR binding regions of the TetR repressible promoter (R0040). The promoter therefore should not bind TetR is not repressible. The presence of aTc or other TetR ligands should not influence transcription of RFP. We will use it as a negative control when using repClone (J100205) to test the effects of modifications to the promoter.  
  
 
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Revision as of 19:45, 3 December 2016


Scrambled TetR repressible promoter

We scrambled the TetR binding regions of the TetR repressible promoter (R0040). The promoter therefore should not bind TetR is not repressible. The presence of aTc or other TetR ligands should not influence transcription of RFP. We will use it as a negative control when using repClone (J100205) to test the effects of modifications to the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]