Difference between revisions of "Part:BBa J100311:Design"
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===Design Notes=== | ===Design Notes=== | ||
We were cautious to scramble only those bases involved in TetR binding, while keeping other bases the same in order to maintain as best we can the spacing and structure of the original promoter. | We were cautious to scramble only those bases involved in TetR binding, while keeping other bases the same in order to maintain as best we can the spacing and structure of the original promoter. | ||
| − | + | The resulting promoter is one base shorter than the original as a cytosine was lost during assembly. However, we accepted this mutation because it was located in the second scrambled binding region and the promoter functioned as expected when inserted in repClone (see J100312). | |
Latest revision as of 17:01, 2 December 2016
Scrambled TetR repressible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We were cautious to scramble only those bases involved in TetR binding, while keeping other bases the same in order to maintain as best we can the spacing and structure of the original promoter. The resulting promoter is one base shorter than the original as a cytosine was lost during assembly. However, we accepted this mutation because it was located in the second scrambled binding region and the promoter functioned as expected when inserted in repClone (see J100312).
Source
The original sequence is the TetR repressible promoter, R0040. Binding regions were identified from Orth P, et al., "Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system." DOI: 10.1038/73324