Difference between revisions of "Part:BBa J100204"

Revision as of 21:33, 5 November 2016

actClone Red

We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and a DNA segment that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
Illegal AgeI site found at 1612
Illegal AgeI site found at 1724
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 928
Illegal BsaI.rc site found at 817
Illegal SapI site found at 708

@@ Line 4: / Line 4: @@
 We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and a DNA segment that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
-The OmpR promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription.
+The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription.
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