Difference between revisions of "Part:BBa K2012027"

Latest revision as of 14:57, 1 November 2016

PcpcG2-172-B0034-PleD-His(6)Tag

PcpcG2-172 is a 172bp modified green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K2012015).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified through Ni-chelating affinity chromatography.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 212
Illegal BamHI site found at 1370
Illegal XhoI site found at 1564
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 628
Illegal NgoMIV site found at 775
Illegal AgeI site found at 1111
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 285

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 <partinfo>BBa_K2012027 short</partinfo>
-PcpcG2-172 is a 172bp modified green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K2012015).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified through Ni-chelating affinity chromatography..
+PcpcG2-172 is a 172bp modified green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K2012015).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified through Ni-chelating affinity chromatography.
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