Difference between revisions of "Part:BBa K2012025"
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<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><img height="318.92999267578125" src="https://static.igem.org/mediawiki/2016/5/5f/Eeeee.jpeg" width="480.0"></span><span style="font-family:Calibri"><span></span></span></p> | <div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><img height="318.92999267578125" src="https://static.igem.org/mediawiki/2016/5/5f/Eeeee.jpeg" width="480.0"></span><span style="font-family:Calibri"><span></span></span></p> | ||
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"> </span></p> | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"> </span></p> | ||
| − | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as </span><span style="font-family:Calibri">chromophore</span><span style="font-family:Calibri">. </span><span style="font-family:Calibri"><span></span></span></p> | + | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><b>Figure 1.</b> Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as </span><span style="font-family:Calibri">chromophore</span><span style="font-family:Calibri">. </span><span style="font-family:Calibri"><span></span></span></p> |
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">F</span><span style="font-family:Calibri">luorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. </span><span style="font-family:Calibri">PcpcG2-172 </span><span style="font-family:Calibri">shows high efficiency.<span></span></span></p> | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">F</span><span style="font-family:Calibri">luorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. </span><span style="font-family:Calibri">PcpcG2-172 </span><span style="font-family:Calibri">shows high efficiency.<span></span></span></p> | ||
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">More importantly, l</span><span style="font-family:Calibri">eaked expression in darkness significantly reduced after truncation of the constitutive promoter. </span><span style="font-family:Calibri;font-weight:bold"><span></span></span></p> | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">More importantly, l</span><span style="font-family:Calibri">eaked expression in darkness significantly reduced after truncation of the constitutive promoter. </span><span style="font-family:Calibri;font-weight:bold"><span></span></span></p> | ||
Latest revision as of 14:55, 1 November 2016
PcpcG2-172-B0034-sfGFP
sfGFP Generator from pJT119b original plasmid.
Figure 1. Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.
Fluorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. PcpcG2-172 shows high efficiency.
More importantly, leaked expression in darkness significantly reduced after truncation of the constitutive promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]