Difference between revisions of "Part:BBa K2012017"
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<partinfo>BBa_K2012017 short</partinfo>
 
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this generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23113,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength  with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]
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<p>The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids</p>
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This generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23113,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength  with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]
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<html><img src="https://static.igem.org/mediawiki/parts/0/00/T--HZAU-China--swarming_plate_of_CheZ.jpg" style="width:900px;margin-left:0%;"/></html>
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<img src="https://static.igem.org/mediawiki/parts/0/00/T--HZAU-China--swarming_plate_of_CheZ.jpg" style="width:900px;margin-left:0%;"/>
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<p><b>Figure 1.</b> The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids.</p>
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Revision as of 14:35, 1 November 2016


A strong generator of CheZ

This generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23113,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]


Figure 1. The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]