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| − | ===Improvements===
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| − | <p><b>Group:</b> <a href="http://2016.igem.org/Team:TJUSLS_China" target="_blank">TJUSLS_China 2016</a></p>
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| − | <p><b>Author:</b> Zhuozhi Chen</p>
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| − | <b>Summary:</b>inJanus-linker-GCW61 is a construction we designed, which aims to change the hydrophobicity of the outer surface of the cell wall. GCW61 is an anchoring protein derived from Pichia GS115. When fused with a certain protein on its C-terminus, such as inJanus, this protein can be displayed on to the cell wall. Thus, inJanus, a class I hydrophobin, can eventually manage to increase the hydrophobicity of the cell wall, having displayed on cell surface, and ultimately help in targeting hydrophobic substrates when the host cell is a whole cell biocatalyst. Another usage of this construct is to protect the host cell from severe or extreme environments, since hydrophobins are considered to be very stable and protective.<br>
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| − | We co-displayed PETase-linker-GCW21/51/61 along with inJanus-linker-GCW61, in attempt to make our whole cell biocatalyst more efficient and tolerant. Previous studies on co-displaying hydrophobin with lipases have already shown positive results on the promotion of efficiency. It is confirmed that most lipases are more active when entering a more hydrophobic environment. Since PETase is also classified as lipase, we decided to build this construction and make this attempt. <br>
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| − | <b>New parts:</b> <partinfo>K1921026</partinfo>.
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| − | ===Co-display HPLC Results===
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| − | <p style="text-align: center;">
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| − | https://static.igem.org/mediawiki/igem.org/b/b9/ProofTJU19.jpg<br>
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| − | '''Figure 1.''' The activity of the first group of ppic9-PETase-linker-GCW51 & ppiczaA-inJanus-linker-GCW61 co-display transformants in different hours and amount of yeast.<br>
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| − | </p>
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| − | <p style="text-align: center;">
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| − | https://static.igem.org/mediawiki/igem.org/a/a0/ProofTJU20.jpg<br>
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| − | '''Figure 2.''' The activity of the second group of ppic9-PETase-linker-GCW51 & ppiczaA-inJanus-linker-GCW61 co-display transformants in different hours and amount of yeast.<br>
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| − | </p>
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| − | </html>
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| − | Concluding, we successfully improved this part by constructing and characterizing a new BioBricks based on this part. More information on these parts can be found here: <partinfo>K1921026</partinfo>.
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| − | <h2>References</h2>
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| − | [1] Shosuke Y, Kazumi H, Toshihiko T, Ikuo Taniguchi, Hironao Y, Yasuhito M, Kiyotsuna T, Kenji M, Yoshiharu K, Kohei O. 2016. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science 351, 1196-1199. DOI: 10.1126/science.aad6359.<br>
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| − | [2] Pan W, Jie H, Yufei S, Matthew R, Li Z, Shuangyan H, Shuli L, Haixin S, Ying L. 2016. Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B. Appl Microbiol Biotechnol DOI 10.1007/s00253-016-7431-x<br>
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| − | [3] Brzozowski A, Derewenda U, Derewenda Z, Dodson G, Lawson D, Turkenburg J, Bjorkling F, Huge-Jensen B, Patkar S, Thim L . 1991. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 351(6326):491–494. doi:10.1038/351491a0<br>
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