Difference between revisions of "Part:BBa K2145124:Experience"
| Line 6: | Line 6: | ||
===Applications of BBa_K2145124=== | ===Applications of BBa_K2145124=== | ||
[[File:Splates95102.png]]<br> | [[File:Splates95102.png]]<br> | ||
| + | Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG99 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR. | ||
[[File:Alverno_ca_99.png]] | [[File:Alverno_ca_99.png]] | ||
Revision as of 00:18, 31 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2145124
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG99 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
User Reviews
UNIQ8d9eab2c71e2b5cb-partinfo-00000000-QINU UNIQ8d9eab2c71e2b5cb-partinfo-00000001-QINU