Difference between revisions of "Part:BBa K2145105:Experience"

Revision as of 00:16, 31 October 2016

Applications of BBa_K2145105

Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG98 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

User Reviews

UNIQafdda86209e8a9bf-partinfo-00000000-QINU UNIQafdda86209e8a9bf-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
 ===Applications of BBa_K2145105===
 [[File:Splates95102.png]]<br>
+Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG98 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
 [[File:Alverno_ca_98.png]]