Difference between revisions of "Part:BBa K2145104:Experience"

Revision as of 00:06, 31 October 2016

This experience page is provided so that any user may enter their experience using this part.

Applications of BBa_K2145104

Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

User Reviews

UNIQ7c15969872bc0c2d-partinfo-00000000-QINU UNIQ7c15969872bc0c2d-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>
-Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
 ===Applications of BBa_K2145104===
 [[File:Splates95102.png]]<br>
+Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
 [[File:Alverno_ca_97.png]]