Difference between revisions of "Part:BBa K2145001"

 
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This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.  
 
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.  
 +
Direction of GFP: Forward, toward the spacer
 +
Direction of RFP: Forward, away from the spacer
  
 
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Revision as of 23:12, 30 October 2016


GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins. Direction of GFP: Forward, toward the spacer Direction of RFP: Forward, away from the spacer

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2270
    Illegal AgeI site found at 2382
  • 1000
    COMPATIBLE WITH RFC[1000]