Difference between revisions of "Part:BBa K2139017"

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Cellulomonas fimi uses 3 endoglucanases (including CenA, accession M15823) and an exoglucanase in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose. This part was codon optimized for the expression in Caulobacter crescentus and synthesized by IDT.  
 
Cellulomonas fimi uses 3 endoglucanases (including CenA, accession M15823) and an exoglucanase in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose. This part was codon optimized for the expression in Caulobacter crescentus and synthesized by IDT.  
  
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We improved the characterization of this part by optimizing the expression and attempted to express the enzyme on the surface layer of <i>C.crescentus</i>.
===Usage and Biology===
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[[Image:British_Columbia.jpg|300px]]</center>
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Revision as of 22:02, 30 October 2016


Coding Sequence of CenA

Cellulomonas fimi uses 3 endoglucanases (including CenA, accession M15823) and an exoglucanase in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose. This part was codon optimized for the expression in Caulobacter crescentus and synthesized by IDT.

We improved the characterization of this part by optimizing the expression and attempted to express the enzyme on the surface layer of C.crescentus.

British Columbia.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]