Difference between revisions of "Part:BBa K1961006"
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We redo ligation and do function assay. | We redo ligation and do function assay. | ||
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| + | We tested the survival of E. coli transformed with K1961006 in the Pb<sup>2+</sup> | ||
[[File: HSiTAIWAN_Pb_Growth_Curve.jpeg|600px|thumb|left|alt text]] | [[File: HSiTAIWAN_Pb_Growth_Curve.jpeg|600px|thumb|left|alt text]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:00, 30 October 2016
Lead ion detector
This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+ - PbrR in the presence of Pb2+ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.
We redo ligation and do function assay.
We tested the survival of E. coli transformed with K1961006 in the Pb2+
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1281
Illegal AgeI site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 686
HSiTAIWAN iGEM16