Difference between revisions of "Part:BBa K1961006"

Revision as of 20:59, 30 October 2016

Lead ion detector

This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb²⁺ - PbrR in the presence of Pb²⁺ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb²⁺ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.

We redo ligation and do function assay.

alt text

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1281
Illegal AgeI site found at 1393
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 686

HSiTAIWAN iGEM16

@@ Line 6: / Line 6: @@
 (1) PbrR generation:this part produces PbrR which can form a dimer of Pb<sup>2+</sup> - PbrR in the presence of Pb<sup>2+</sup> .
 (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb<sup>2+</sup> /PbrR.  RFP expression can be observed when the dimer binds onto the promoter.
 We redo ligation and do function assay.
+[[File: HSiTAIWAN_Pb_Growth_Curve.jpeg|600px|thumb|left|alt text]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===