Difference between revisions of "Part:BBa K2139011"

Latest revision as of 20:18, 30 October 2016

E1 with Terminator

E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the crystaline cellulose chain. It can be used in conjunction with beta-1,4-exoglucanases and beta-1,4-glucosidase for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for Caulobacter crescentus and synthasized by IDT.

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2139011 short</partinfo>
-E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the crystaline cellulose chain. It can be used in conjunction with beta-1,4-exoglucanases and beta-1,4-glucosidase for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for Caulobacter crescentus.
+E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the crystaline cellulose chain. It can be used in conjunction with beta-1,4-exoglucanases and beta-1,4-glucosidase for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for Caulobacter crescentus and synthasized by IDT.
 Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full