Difference between revisions of "Part:BBa K1961005"
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We tested the survival of E.coli in tetracycline(TC).  E. coli can survival in TC of 1000 ng/ml.  In the following figure, it shows that GFP expression of autoregulation is slower than that with constitutive promoter(BBa_K1961005)
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We tested the survival of E.coli in tetracycline(TC).  E. coli can survival in TC of 1000 ng/ml.  In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)
  
 
[[File: HSiTAIWAN_Autoregulation_Table.jpeg|600px|thumb|left|alt text]]
 
[[File: HSiTAIWAN_Autoregulation_Table.jpeg|600px|thumb|left|alt text]]

Revision as of 20:01, 30 October 2016


PbrR generator expressed by GFP under control of general promoter (J23102)

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation.

alt text


We tested the survival of E.coli in tetracycline(TC). E. coli can survival in TC of 1000 ng/ml. In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)

alt text


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111