Difference between revisions of "Part:BBa I730013"
Line 2: Line 2:
 
<partinfo>BBa_I730013 short</partinfo>
 
<partinfo>BBa_I730013 short</partinfo>
  
−
pOmpRm-ptetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator  
+
pOmpRm-ptetR-strong RBS-LacI repressor(LVA+)-strong RBS-CFP(AAV+)-terminator  
  
 
R0083.R0011.B0034.C0040.E0422  
 
R0083.R0011.B0034.C0040.E0422  

Revision as of 16:39, 11 October 2007

pOmpRm-pLacI-TetR repressor-CFP

pOmpRm-ptetR-strong RBS-LacI repressor(LVA+)-strong RBS-CFP(AAV+)-terminator

R0083.R0011.B0034.C0040.E0422

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing mutated promoter for the OmpC gene, as the input of the device. this mutant is said to (and characterised as) be weaker than regular pOmpR

as an output we have thought of the fluorescence protein CFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]