Difference between revisions of "Part:BBa K2165004"

Revision as of 03:19, 30 October 2016

CUP1 yeast inducible promoter with RFC[10] restriction sites removed

The Cup1 promoter enables binding of RNA Polymerase II and the subsequent transcription of downstream DNA to mRNA. It is activated by ACE1, a transcription factor which binds to copper ions. It was previously available as a standalone part as BBa_K945002, produced by Tec-Monterrery’s 2012 iGEM team; however, the original part contained numerous illegal restriction sites preventing its usability in standard biobrick assembly. Hard information of the Greenfield 2011 team’s BBa_K586000 claims the part is the Cup1 regulatory region, but based on the sequence and its twin we believe it to be mislabeled and that it is instead the CupI CDS.

We acquired our starting sequence from the Vicker’s Lab at the Australian Institute for Bioengineering and Nanotechnology via Addgene, subsequently removed or altered bases corresponding to illegal cut sites, added a biobrick prefix and suffix, and finally ordered the unit as a geneblock from IDT.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2165004 short</partinfo>
-CUP1 is a commonly used inducible transcription promoter for yeast that leads to enhanced expression of its respective gene(s) in the presence of cupric ions.  The documented natural variants of the CUP1 promoter, including part K945002 in the registry (https://parts.igem.org/Part:BBa_K945002), contain RFC[10] illegal restriction sites that have been eliminated in this part so that it may be used in other biobricks.
+The Cup1 promoter enables binding of RNA Polymerase II and the subsequent transcription of downstream DNA to mRNA. It is activated by ACE1, a transcription factor which binds to copper ions. It was previously available as a standalone part as [https://parts.igem.org/Part:BBa_K945002 BBa_K945002], produced by Tec-Monterrery’s 2012 iGEM team; however, the original part contained numerous illegal restriction sites preventing its usability in standard biobrick assembly. Hard information of the Greenfield 2011 team’s [https://parts.igem.org/Part:BBa_K586000 BBa_K586000] claims the part is the Cup1 regulatory region, but based on the sequence and its twin we believe it to be mislabeled and that it is instead the CupI CDS.
+We acquired our starting sequence from the Vicker’s Lab at the Australian Institute for Bioengineering and Nanotechnology via Addgene, subsequently removed or altered bases corresponding to illegal cut sites, added a biobrick prefix and suffix, and finally ordered the unit as a geneblock from IDT.
 <!-- Add more about the biology of this part here