Difference between revisions of "Part:BBa K1947002"
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<partinfo>BBa_K1947002 parameters</partinfo> | <partinfo>BBa_K1947002 parameters</partinfo> | ||
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+ | ===References=== | ||
+ | Telenti A, Southworth M, Alcaide F et al(1997) The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal. J Bacteriol 179:6378–6382 |
Latest revision as of 03:14, 30 October 2016
Intein
This sequence encodes a short polypeptide which can be specifically recognized and spliced by DL-Dithiothreitol(DTT). In our case we add this short sequence between the recombinant protein which we want to purify and the linker protein Spytag, in order to separate the recombinant protein form the Magnetosome.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 517
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 437
References
Telenti A, Southworth M, Alcaide F et al(1997) The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal. J Bacteriol 179:6378–6382