Difference between revisions of "Part:BBa I730011"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I730011 short</partinfo>
 
<partinfo>BBa_I730011 short</partinfo>
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pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator
 
pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator
  
R0082.R0040.B0034.C0012.E0422
+
R0082.R0040.B0034.C0012.E0434
  
 
intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.  
 
intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.  

Revision as of 16:00, 11 October 2007

pOmpR-ptetR-LacI repressor-YFP

pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator

R0082.R0040.B0034.C0012.E0434

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.

as an output we have thought of the fluorescence protein CFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]