Difference between revisions of "Part:BBa K2179003"
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| − | <h5>Translational Unit for Chlorite Dismutase</h5> | + | <h5>Translational Unit for Chlorite Dismutase (Cld)</h5> |
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Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion. | Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion. | ||
Revision as of 23:43, 29 October 2016
Translational Unit for Chlorite Dismutase (Cld)
Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 807
Illegal BsaI.rc site found at 6