Difference between revisions of "Part:BBa K1962005"

Revision as of 23:33, 29 October 2016

Immunity Protein Im-E9

This biobrick codes for the Immunity Protein that is specific for Colicin E9. This protein is able to protect a host bacterial cell against attack by Colicin E9 since it binds tightly and specifically to the DNase domain of E9 and inhibits its bactericidal activity.

•••••

Parts Collection 2016

This is part of a Part Collection of 17 BioBricks designed by Dundee iGEM 2016. This collection will be useful to teams working with toxins as we have submitted new toxins to the registry. Working with bacterial toxins is difficult due to the risk of toxicity to the chassis, so the corresponding immunity for our toxins were also submitted. We have also submitted these toxins lacking their cytotoxic domains replacing it with a multiple cloning site which will allow for different toxic domains to be fused at the C-terminus and thereby generating a synthetic toxin. In addition, there are three well-characterised promoters that can be used to initiate gene expression at various points in the digestive tract, to enable devices to function within a human or animal. Finally, a lysis cassette was constructed to lyse or burst cells, thus releasing the toxins and destroying the GM bacteria to prevent its release to the environment.

This BBa_K1962005 is an immunity protein against colicin E9, which is not currently in The Registry. This part will be useful for teams wishing to clone colicin E9 in the future, helping to neutralise the toxin during cloning of the gene

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This biobrick codes for the Immunity Protein that is specific for Colicin E9. This protein is able to protect a host bacterial cell against attack by Colicin E9 since it binds tightly and specifically to the DNase domain of E9 and inhibits its bactericidal activity.
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_K892008 AddReview 5</partinfo>
+<I>Parts Collection 2016</I>
+|width='60%' valign='top'|
+This is part of a Part Collection of 17 BioBricks designed by Dundee iGEM 2016. This collection will be useful to teams working with toxins as we have submitted new toxins to the registry. Working with bacterial toxins is difficult due to the risk of toxicity to the chassis, so the corresponding immunity for our toxins were also submitted. We have also submitted these toxins lacking their cytotoxic domains replacing it with a multiple cloning site which will allow for different toxic domains to be fused at the C-terminus and thereby generating a synthetic toxin. In addition, there are three well-characterised promoters that can be used to initiate gene expression at various points in the digestive tract, to enable devices to function within a human or animal. Finally, a lysis cassette was constructed to lyse or burst cells, thus releasing the toxins and destroying the GM bacteria to prevent its release to the environment.
+This <partinfo>BBa_K1962005</partinfo> is an immunity protein against colicin E9, which is not currently in The Registry. This part will be useful for teams wishing to clone colicin E9 in the future, helping to neutralise the toxin during cloning of the gene
+|}