Difference between revisions of "Part:BBa K1937002"
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This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France). | This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France). | ||
| − | More information available on the <a href= | + | More information available on the <a href= "https://parts.igem.org/Part:BBa_K1937002:Design"> design page of the Registry </a> and at http://2016.igem.org/Team:Toulouse_France/Description |
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| − | ===Usage and Biology | + | ===Usage and Biology== |
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Revision as of 19:52, 29 October 2016
OriKan: a composite part to turn any pSB1C3 into a Bacillus plasmid
Part: BBa_K1937002 (pSB1C3-OriKan)
(Chassis E. coli/B. subtilis, carrier plasmid pSB1C3)
Length: 2510 bp
While Bacillus subtilis is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they are registered after sub-cloning in the E. coli plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (E. coli/B. subtilis). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).
More information available on the <a href= "https://parts.igem.org/Part:BBa_K1937002:Design"> design page of the Registry </a> and at http://2016.igem.org/Team:Toulouse_France/Description
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2196
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1807
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 522
Illegal SapI.rc site found at 2020