Difference between revisions of "Part:BBa K1893032"
 
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<partinfo>BBa_K1893032 short</partinfo>
 
<partinfo>BBa_K1893032 short</partinfo>
  
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Reporter protein superfolder GFP (SFGFP) as used in the paper Chappell et al., 2010.  
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The reporter gene super folder GFP (SFGFP) that was used by Chappell et al. (2015) in the initial design of the STAR system. We decided to use this SFGFP sequence for our STAR part as well, since it’s compatibility with the STAR sequence was validated by Chapell et al. (2015).
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 16:41, 29 October 2016


Superfolder GFP (SF-GFP)

The reporter gene super folder GFP (SFGFP) that was used by Chappell et al. (2015) in the initial design of the STAR system. We decided to use this SFGFP sequence for our STAR part as well, since it’s compatibility with the STAR sequence was validated by Chapell et al. (2015).

Usage and Biology

Excitation wavelength in 485 nm and emission wavelength is 520nm. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]