Difference between revisions of "Part:BBa K1893011"

 
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<partinfo>BBa_K1893011 short</partinfo>
 
<partinfo>BBa_K1893011 short</partinfo>
  
This is the Anti-STAR sequence under control of the constitutive Anderson promoter j23119. Anti-STAR is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it is fully complementary and thus binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.
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This is the Anti-STAR sequence (BBa_K1893014) under control of the constitutive Anderson promoter j23119. Anti-STAR is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it is fully complementary and thus binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.
  
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===Usage and Biology===
 
===Usage and Biology===
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The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.
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Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.
  
 
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Latest revision as of 16:31, 29 October 2016


Constitutive anti-STAR (J23119+anti-STAR)

This is the Anti-STAR sequence (BBa_K1893014) under control of the constitutive Anderson promoter j23119. Anti-STAR is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it is fully complementary and thus binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.

Usage and Biology

The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.

Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]