Difference between revisions of "Part:BBa K1952012:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
Utilized DNA from construction of K1952002 together with other spacer DNA and the coding region of LacZ alpha (for Blue-White screening) in Gibson assembly. Interchangable with parts for K1952009 assembly.
  
  
  
−
===Source===
 
  
−
Hydrazine Synthase subunit alpha (Kust-2861) with strong RBS under lactose-inducible promoter,
+
===Source===
  
−
followed by LacZ alpha subunit reporter gene.
+
DNA synthesized by Integrated DNA technologies.
  
 
===References===
 
===References===
 +
Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. ''Nature'' Vol 479 [doi:10.1038/nature10453]

Latest revision as of 15:53, 29 October 2016


Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Utilized DNA from construction of K1952002 together with other spacer DNA and the coding region of LacZ alpha (for Blue-White screening) in Gibson assembly. Interchangable with parts for K1952009 assembly.



Source

DNA synthesized by Integrated DNA technologies.

References

Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]