Difference between revisions of "Part:BBa K1952010"
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Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion). | Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion). | ||
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| + | Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal. | ||
Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells. | Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells. | ||
Revision as of 15:48, 29 October 2016
Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter
Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).
Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.
Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 445
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 407
Illegal XhoI site found at 1474 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 568
Illegal AgeI site found at 1461 - 1000COMPATIBLE WITH RFC[1000]