Difference between revisions of "Part:BBa K1952010"
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.
  
 
<!-- -->
 
<!-- -->

Revision as of 15:47, 29 October 2016


Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter

Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]