Difference between revisions of "Part:BBa K1952009"

Latest revision as of 15:40, 29 October 2016

Hydrazine Synthase subunit operon

Lac-promoter driven operon, with three separate open reading frames representing K. stuttgartiensis Hydrazine Synthase multi-subunit enzyme. (Subunits gamma, beta, and alpha, respectively.)

Expression or activity not yet confirmed.

This enzyme is a key step in the anaerobic ammonia oxidation pathway, which converts ammonia (NH4) and nitric oxide (NO) into nitrogen gas (N2). The Hydrazine Synthase enzyme takes the two starting reactants and produces the intermediate hydrazine (N2H4).

For more information see Kartal, B., Keltjens, J., (2016) Anammox Biochemistry: A Tale of Two Heme c proteins. Trends in Biochemical Sciences. [http://dx.doi.org/10.1016/j.tibs.2016.08.015]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2297
Illegal BamHI site found at 966
Illegal BamHI site found at 1766
Illegal BamHI site found at 1967
Illegal BamHI site found at 2090
Illegal BamHI site found at 4528
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3583
Illegal AgeI site found at 2434
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1443

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1952009 short</partinfo>
-Protein coding region with translation initiation signals, to be used alone or in operon
+Lac-promoter driven operon, with three separate open reading frames representing K. stuttgartiensis Hydrazine Synthase multi-subunit enzyme. (Subunits gamma, beta, and alpha, respectively.)
+Expression or activity not yet confirmed.
+This enzyme is a key step in the anaerobic ammonia oxidation pathway, which converts ammonia (NH4) and nitric oxide (NO) into nitrogen gas (N2). The Hydrazine Synthase enzyme takes the two starting reactants and produces the intermediate hydrazine (N2H4).
+For more information see Kartal, B., Keltjens, J., (2016) Anammox Biochemistry: A Tale of Two Heme c proteins. Trends in Biochemical Sciences. [http://dx.doi.org/10.1016/j.tibs.2016.08.015]
 <!-- Add more about the biology of this part here