Difference between revisions of "Part:BBa K1952002:Design"

 
Line 6: Line 6:
  
  
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===Design Notes===
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===Design===
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hydrazine synthase subunit
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This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.
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+
  
  
 
===Source===
 
===Source===
  
−
hydrazine synthase subunit
+
K. stuttgartiensis protein sequence; GenBank CAJ73612.1 [https://www.ncbi.nlm.nih.gov/protein/91200563]
 +
 
 +
The DNA for this part was synthesized by Integrated DNA technologies in two separate DNA fragments with overlap.
  
 
===References===
 
===References===
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 +
Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. ''Nature'' Vol 479 [doi:10.1038/nature10453]

Revision as of 15:33, 29 October 2016


Hydrazine Synthase alpha subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1233
    Illegal AgeI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.


Source

K. stuttgartiensis protein sequence; GenBank CAJ73612.1 [1]

The DNA for this part was synthesized by Integrated DNA technologies in two separate DNA fragments with overlap.

References

Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]