Difference between revisions of "Part:BBa K1952000:Design"

 
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===Design Notes===
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===Design===
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design
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This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.
  
  
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===Source===
 
===Source===
  
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source
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K. stuttgartiensis protein sequence; GenBank CAJ73611.1 [https://www.ncbi.nlm.nih.gov/protein/91200562]
  
 
===References===
 
===References===
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 +
Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. ''Nature'' Vol 479 [doi:10.1038/nature10453]

Revision as of 15:31, 29 October 2016


Hydrazine Synthase gamma subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.


Source

K. stuttgartiensis protein sequence; GenBank CAJ73611.1 [1]

References

Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]