Difference between revisions of "Part:BBa K2165002"

 
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<partinfo>BBa_K2165002 short</partinfo>
 
<partinfo>BBa_K2165002 short</partinfo>
  
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This part contains the VioC gene codon optimized for yeast (BBa_K2165000) attached to the CUP1 inducible yeast promoter (BBa_K2165004) and the ADH1 terminator. The inclusion of an inducible promoter in this construct makes it easier to control the color output of the violacein pathway by altering the concentration of cupric ions in the cell environment to control the level of expression in the presence of other genes in the violacein pathway.  
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<p>This biobrick utilizes our Cup1 promoter. Following the binding of a heavy metal to the Cup1 promoter then VioC transcription is upregulated. The effects on the transcription of the VioC protein can be seen above in the VioC section. The biobrick ends with the ADH1 terminator [https://parts.igem.org/Part:BBa_K801012 BBa_K801012] for yeast that was submitted by the TU Munich team in 2012.</p>
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<p>This biobrick was designed to characterize the codon optimized version of VioC. The codon optimized VioC should have higher translation rates than the previously made VioC, and this plasmid construct will be used to determine the change in the expression levels for the pathway. This composite part was also designed for use in the University of Washington 2016 iGEM team's chemostat-autoinjector proof of concept. For more information on the role of VioC in the violacein pathway see part [https://parts.igem.org/Part:BBa_K2165000 BBa_K2165000].</p>
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<p>It should be stressed that without the other main components of the Violacein pathway (VioA, VioB, and VioE, see part [https://parts.igem.org/Part:BBa_K726015 BBa_K726015] as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.</p>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2165002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2165002 SequenceAndFeatures</partinfo>

Revision as of 13:07, 29 October 2016


Violacein C gene with CUP1 promoter + ADH1 terminator

This biobrick utilizes our Cup1 promoter. Following the binding of a heavy metal to the Cup1 promoter then VioC transcription is upregulated. The effects on the transcription of the VioC protein can be seen above in the VioC section. The biobrick ends with the ADH1 terminator BBa_K801012 for yeast that was submitted by the TU Munich team in 2012.

This biobrick was designed to characterize the codon optimized version of VioC. The codon optimized VioC should have higher translation rates than the previously made VioC, and this plasmid construct will be used to determine the change in the expression levels for the pathway. This composite part was also designed for use in the University of Washington 2016 iGEM team's chemostat-autoinjector proof of concept. For more information on the role of VioC in the violacein pathway see part BBa_K2165000.

Usage and Biology

It should be stressed that without the other main components of the Violacein pathway (VioA, VioB, and VioE, see part BBa_K726015 as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 370