Difference between revisions of "Part:BBa K2066109"

 
 
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<partinfo>BBa_K2066109 short</partinfo>
 
<partinfo>BBa_K2066109 short</partinfo>
  
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This part places both pTet GFP and it's repressor TetR on the same plasmid, which both reduces metabolic stress on transformed cells and helps limit the number of plasmids used by the circuit control toolbox. Placing all circuit control parts on a single plasmid will allow a workable number of plasmids to be used for the input circut to the circuit control toolbox circuit.
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This part combines a pTet GFP reporter (BBa_K2066023) and a constitutive tetR expression construct (BBa_K2066022) onto the same plasmid, with a modified RBS on the tetR expression construct in order to replicate the effect of having the tetR construct be on a lower-copy plasmid.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 06:13, 29 October 2016


pTet GFP + TetR (weakened)

This part combines a pTet GFP reporter (BBa_K2066023) and a constitutive tetR expression construct (BBa_K2066022) onto the same plasmid, with a modified RBS on the tetR expression construct in order to replicate the effect of having the tetR construct be on a lower-copy plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1004
    Illegal NheI site found at 1027
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764