Difference between revisions of "Part:BBa K2066033"
 
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<partinfo>BBa_K2066033 short</partinfo>
 
<partinfo>BBa_K2066033 short</partinfo>
  
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This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 and UNS3.
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This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:20, 29 October 2016


pBad - RFP on UNS

This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001