Difference between revisions of "Part:BBa K2066028"

Latest revision as of 03:13, 29 October 2016

pBAD - LacI/Mcherry fusion

This is a device which expresses an arabinose-inducible lacI-mCherry fusion protein, in order to simultaneously repress downstream genes while quantifying the level of repressor concentration. The part is on the UNS backbone standard designed by William and Mary iGEM 2016, so the insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1245
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1184
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1019
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1001

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2066028 short</partinfo>
-This is a Mcherry reporter protein fused to a functional LacI repressor under the control of the arabinose inducible promoter pBAD on the UNS backbone. This part was originally designed to be used as an upstream circuit simulator for WM16_iGEM's circuit control toolbox. The inducible pBad represents an arbitrary circuit with LacI as the product, and as the input to the orthogonal circuit used to modify the transfer function. The mcherry allows for the quantification of the input in the same cell as the quantification of the output (GFP).
+This is a device which expresses an arabinose-inducible lacI-mCherry fusion protein, in order to simultaneously repress downstream genes while quantifying the level of repressor concentration. The part is on the UNS backbone standard designed by William and Mary iGEM 2016, so the insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019).
 <!-- Add more about the biology of this part here