Difference between revisions of "Part:BBa K2066055"
 
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<partinfo>BBa_K2066055 short</partinfo>
 
<partinfo>BBa_K2066055 short</partinfo>
  
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This part will let us express the entire promoter characterization without riboJ construct in vitro using a T7 in vitro transcription kit, letting us assess the ability of broccoli to fluoresce in the genetic context of our part. Results should be compared to K2066054, which is the same as this part except with the RiboJ sequence.  
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This part is part of a library of parts designed to characterize the influence of RiboJ on the absolute gene expression level on various promoters, including the entire Anderson Promoter Library.
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The part follows the UNS standard developed by William and Mary iGEM 2016, in which the insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) for ease of cloning via Gibson or Golden Gate Assembly.
 
   
 
   
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:40, 29 October 2016


T7 Promoter without RiboJ Promoter Characterization Part

This part is part of a library of parts designed to characterize the influence of RiboJ on the absolute gene expression level on various promoters, including the entire Anderson Promoter Library.

The part follows the UNS standard developed by William and Mary iGEM 2016, in which the insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) for ease of cloning via Gibson or Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]