Difference between revisions of "Part:BBa K2066508"

Latest revision as of 02:26, 29 October 2016

Modified pLacO-1 Promoter (Lou et. al 2012)

This is a modified version of the plLacO-1 promoter used in Lou et al. 2012. Modifications were minor and performed to standardize the plLacO-1 sequence with other sequences in the literature.

Lou, C., Stanton, B., Chen, Y. J., Munsky, B., & Voigt, C. A. (2012). Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nature biotechnology, 30(11), 1137-1142.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2066508 short</partinfo>
-This promoter sequence is modified from section V of Supplementary Material of Lou et al. The Supplementary sequence contains 98bp of the end of BioBrick backbone pSB1C3, followed by an EcoRI site and an XbaI site, then 20bp of the beginning of pTac (as described in fig. S1),  before beginning the sequence of plLacO-1 (as described in fig. S1). Here we use only the 20bp of pTac followed by the plLacO-1 sequence as described in fig. S1. WM iGEM 2016 used this part for our Ribozyme Characterization project.
+This is a modified version of the plLacO-1 promoter used in Lou et al. 2012. Modifications were minor and performed to standardize the plLacO-1 sequence with other sequences in the literature.
+Lou, C., Stanton, B., Chen, Y. J., Munsky, B., & Voigt, C. A. (2012). Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nature biotechnology, 30(11), 1137-1142.
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