Difference between revisions of "Part:BBa K1915001:Experience"
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''Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes.'' | ''Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes.'' | ||
''Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' | ''Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' | ||
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''Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' | ''Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' | ||
| − | Elution 2 exhibited the strongest signal | + | Elution 2 exhibited the strongest signal after visualization of western blot. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:50, 29 October 2016
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Applications of BBa_K1915001
Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction. Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction. Western blot of SDS-PAGE with His antibody. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Elution 2 exhibited the strongest signal after visualization of western blot.
User Reviews
UNIQf950bb1d7dba6c6d-partinfo-00000000-QINU UNIQf950bb1d7dba6c6d-partinfo-00000001-QINU