Difference between revisions of "Part:BBa K1915001:Experience"
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[[File:T--Georgia_State--LT10_Coomassie3.png|none|500px]] | [[File:T--Georgia_State--LT10_Coomassie3.png|none|500px]] | ||
'''Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction.''' | '''Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction.''' | ||
| − | Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. | + | ''Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. |
| − | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column. | + | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' |
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[[File:T--Georgia State--LTNF10 Western.png|none|500px]] | [[File:T--Georgia State--LTNF10 Western.png|none|500px]] | ||
'''Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction.''' | '''Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction.''' | ||
| − | Western blot of SDS-PAGE with His antibody | + | ''Western blot of SDS-PAGE with His antibody |
| − | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column. | + | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.'' |
Elution 2 exhibited the strongest signal | Elution 2 exhibited the strongest signal | ||
Revision as of 01:48, 29 October 2016
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Applications of BBa_K1915001
Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction. Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction. Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Elution 2 exhibited the strongest signal
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