Difference between revisions of "Part:BBa K1915001:Experience"
(→Applications of BBa_K1915001) |
(→Applications of BBa_K1915001) |
||
Line 9: | Line 9: | ||
− | [[File:T--Georgia_State--LT10_Coomassie3.png|200px|Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction.]] | + | [[File:T--Georgia_State--LT10_Coomassie3.png|frame|200px|Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction.]] |
Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. | Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. | ||
Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column. | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column. |
Revision as of 01:40, 29 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1915001
Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Elution 2 exhibited the strongest signal
User Reviews
UNIQaab6734b7dff91bf-partinfo-00000000-QINU UNIQaab6734b7dff91bf-partinfo-00000001-QINU