Difference between revisions of "Part:BBa K1877011"

Revision as of 00:48, 29 October 2016

Tetr Promoter+RBS+GFP+Terminator

The construct is designed to test along with BBa_K1877001. This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch, which serves as a suicide program to prevent bacteria from escaping the laboratory environment and contaminating food and water system. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1877011 short</partinfo>
-This is the pre-testing construct for the second part of our project, but we are not running it alone, and without the presence of Tetr, the construct produces GFP.
+The construct is designed to test along with BBa_K1877001. This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch, which serves as a suicide program to prevent bacteria from escaping the laboratory environment and contaminating food and water system. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration.
 <!-- Add more about the biology of this part here