Difference between revisions of "Part:BBa K1877011:Design"
RuijieTang (Talk | contribs) (→Design Notes) |
RuijieTang (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
The construct is designed to test along with BBa_K1877001. | The construct is designed to test along with BBa_K1877001. | ||
| − | This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration. | + | This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch, which serves as a suicide program to prevent bacteria from escaping the laboratory environment and contaminating food and water system. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration. |
===Source=== | ===Source=== | ||
Latest revision as of 00:35, 29 October 2016
Tetr Promoter+RBS+GFP+Terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724
Design Notes
The construct is designed to test along with BBa_K1877001. This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch, which serves as a suicide program to prevent bacteria from escaping the laboratory environment and contaminating food and water system. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration.
Source
iDT and the registry