Difference between revisions of "Part:BBa K1996004"
 
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<partinfo>BBa_K1996004 short</partinfo>
 
<partinfo>BBa_K1996004 short</partinfo>
  
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Purple chromoprotein
 
  
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Purple chromoprotein
 
===Usage and Biology===
 
===Usage and Biology===
 
Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.<br>
 
Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.<br>
  
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https://static.igem.org/mediawiki/2016/2/21/T--Sydney_Australia--amilCPmutants.png
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<br>
 
<br>
 
<b>Figure 1. Purple, pink and green mutants compared to the wild type.<br>
 
<b>Figure 1. Purple, pink and green mutants compared to the wild type.<br>
 
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.</b><br>
 
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.</b><br>
  
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https://static.igem.org/mediawiki/2016/4/42/T--Sydney_Australia--amilCPspectrum3.png<br>
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<b>Figure 2. Spectrum scan of the extracted protein from the different coloured amilCP mutants. Protein concentration is equal between all samples.</b><br>
 
<b>Figure 2. Spectrum scan of the extracted protein from the different coloured amilCP mutants. Protein concentration is equal between all samples.</b><br>
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https://static.igem.org/mediawiki/2016/6/6e/T--Sydney_Australia--amilCPspectrum4.png<br>
 
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<b>Figure 3. Spectrum scan of the extracted protein from the different coloured amilCP mutants, with the spectrums all adjusted to the same levels to show peaks.</b>
 
  
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<b>Figure 3. Spectrum scan of the extracted protein from the different coloured amilCP mutants, with the spectrums all adjusted to the same levels to show peaks.</b>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:26, 28 October 2016


Purple chromoprotein


Purple chromoprotein ===Usage and Biology=== Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.


Figure 1. Purple, pink and green mutants compared to the wild type.
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.

Figure 2. Spectrum scan of the extracted protein from the different coloured amilCP mutants. Protein concentration is equal between all samples.
Figure 3. Spectrum scan of the extracted protein from the different coloured amilCP mutants, with the spectrums all adjusted to the same levels to show peaks.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]