Difference between revisions of "Part:BBa K1996004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 ( | + | Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.<br> |
| − | https://static.igem.org/mediawiki/2016/2/21/T--Sydney_Australia--amilCPmutants.png<br> | + | <div style = "width: 50%;" > |
| + | https://static.igem.org/mediawiki/2016/2/21/T--Sydney_Australia--amilCPmutants.png | ||
| + | </div> | ||
| + | <br> | ||
<b>Figure 1. Purple, pink and green mutants compared to the wild type.<br> | <b>Figure 1. Purple, pink and green mutants compared to the wild type.<br> | ||
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.</b><br> | Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.</b><br> | ||
Revision as of 17:13, 28 October 2016
Purple chromoprotein
Purple chromoprotein
Usage and Biology
Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.
Figure 1. Purple, pink and green mutants compared to the wild type.
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.
Figure 2. Spectrum scan of the extracted protein from the different coloured amilCP mutants. Protein concentration is equal between all samples.
Figure 3. Spectrum scan of the extracted protein from the different coloured amilCP mutants, with the spectrums all adjusted to the same levels to show peaks.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]