Difference between revisions of "Part:BBa K1893019:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Gp2 sequence was obtained from NCBI (link)
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The gp2 coding sequence was initially obtained by synthesis using the ThermoFisher GeneArt service, based on the T7 phage reference genome provided in the NCBI nucleotide database (NC_001604). The native RBS was replaced with a standard Elowitz RBS [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)] and the resulting sequence was flanked with BioBrick prefix and suffix sequences. The modified coding sequence was PCR amplified from the provided company plasmid cloned into our backbone of choice.
  
 
===Source===
 
===Source===

Latest revision as of 16:33, 28 October 2016


T7 phage gene product 2 (Gp2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


Design Notes

The gp2 coding sequence was initially obtained by synthesis using the ThermoFisher GeneArt service, based on the T7 phage reference genome provided in the NCBI nucleotide database (NC_001604). The native RBS was replaced with a standard Elowitz RBS (BBa_B0034) and the resulting sequence was flanked with BioBrick prefix and suffix sequences. The modified coding sequence was PCR amplified from the provided company plasmid cloned into our backbone of choice.

Source

Gp2 and an RBS was synthesised as a single gene block using the GeneArt® Gene Synthesis service by Thermo Fisher

References