Difference between revisions of "Part:BBa K1976050"

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         <h1>Colicin E2 DNase domain with B0034 RBS</h1>
 
         <h1>Colicin E2 DNase domain with B0034 RBS</h1>
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Colicin DNase domain <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a> with a strong RBS<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
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The colicin DNase domain <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a> is fusioned to the strong ribosomal binding site RBS<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
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    Intermediate for assembly of generators of the Colicin E2 DNase domain.
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This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976026</a>) it serves as a safety mechanism (see part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976054</a>).  
 
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Revision as of 03:16, 28 October 2016

Colicin E2 DNase domain with B0034 RBS

The colicin DNase domain BBa_K1976048 is fusioned to the strong ribosomal binding site RBSBBa_B0034.

Figure 1:Colicin E2 DNase domain based on an MD simulation.


Usage

This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part BBa_K1976026) it serves as a safety mechanism (see part BBa_K1976054).

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007