Difference between revisions of "Part:BBa K1976051"
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        <h1>Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters</h1>
  
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The DNase trypsin fragment (part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976049</a>) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034.
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<partinfo>BBa_K1976051 short</partinfo>
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Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site.
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The DNase trypsin fragment (part 49) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034.
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<b>Figure 1:</b> The structure of the Im2 immunity protein and Colicin E2 in a complex.
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===Usage and Biology===
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This part can be used for assembly of generators. In combination with the immunity protein Im2 (part ?) it serves as a safety mechanism (see part 53).
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===<h2>Usage</h2>===
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This part can be used for assembly of generators. In combination with the immunity protein Im2 (part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976026</a>) it serves as a safety mechanism (see part <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976053</a>).
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===<h2>Characteristics</h2>===
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The DNase trypsin fragment of colicin E2 consists of 137 residues what conforms to about one third to colicin. We used only one of the three domains of colicin since the metabolic burdon would be significant smaller. This fragment differs to the part 50 in 5 additional amino acids since a trypsin digestion described in the literature leaded to this fragment. So we wanted to check out any difference in the activities between the DNase fragment with and without theese additional amino acids. The activity tests showed that the so called miniColicin has functional DNase activity.
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===<h2>Characteristics</h2>===
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Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1976051 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1976051 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
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The DNase trypsin fragment of colicin E2 consists of 137 residues what conforms to about one third to colicin. We used only one of the three domains of colicin since the metabolic burdon would be significant smaller. This fragment differs to the part 50 in 5 additional amino acids since a trypsin digestion described in the literature leaded to this fragment. So we wanted to check out any difference in the activities between the DNase fragment with and without theese additional amino acids. The activity tests showed that the so called miniColicin has functional DNase activity.
 
 
<partinfo>BBa_K1976051 parameters</partinfo>
 
<partinfo>BBa_K1976051 parameters</partinfo>
 
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Revision as of 03:05, 28 October 2016

Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters

The DNase trypsin fragment (part BBa_K1976049) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034.

Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.


Usage

This part can be used for assembly of generators. In combination with the immunity protein Im2 (part BBa_K1976026) it serves as a safety mechanism (see part BBa_K1976053).

Characteristics

The DNase trypsin fragment of colicin E2 consists of 137 residues what conforms to about one third to colicin. We used only one of the three domains of colicin since the metabolic burdon would be significant smaller. This fragment differs to the part 50 in 5 additional amino acids since a trypsin digestion described in the literature leaded to this fragment. So we wanted to check out any difference in the activities between the DNase fragment with and without theese additional amino acids. The activity tests showed that the so called miniColicin has functional DNase activity.

Characteristics

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Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]