Difference between revisions of "Part:BBa K1976051"
Franny3112 (Talk | contribs) |
|||
| Line 4: | Line 4: | ||
Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site. | Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site. | ||
| + | |||
| + | The DNase trypsin fragment (part 49) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This part can be used for assembly of generators. In combination with the immunity protein Im2 (part ?) it serves as a safety mechanism (see part 53). | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| Line 15: | Line 17: | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| + | The DNase trypsin fragment of colicin E2 consists of 137 residues what conforms to about one third to colicin. We used only one of the three domains of colicin since the metabolic burdon would be significant smaller. This fragment differs to the part 50 in 5 additional amino acids since a trypsin digestion described in the literature leaded to this fragment. So we wanted to check out any difference in the activities between the DNase fragment with and without theese additional amino acids. The activity tests showed that the so called miniColicin has functional DNase activity. | ||
<partinfo>BBa_K1976051 parameters</partinfo> | <partinfo>BBa_K1976051 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 02:58, 28 October 2016
Trypsin Fragment of Colicin E2 with B0034 as a RBS
Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site.
The DNase trypsin fragment (part 49) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 64
- 1000COMPATIBLE WITH RFC[1000]